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March 6, 2008

Mtg: The Electronics in Flow Cytometry

by @ 9:09 pm. Filed under ALL, BioEngineering, Electronics Design, Engineering Mgmt, NanoEngineering, Optics/Displays

SCV Consultants’ Network of Silicon Valley
Speaker: William Tynes, Dwayne Yount, Becton Dickinson and Co.
Time: Networking at 7:00 PM, followed by Presentation
Cost: none
Place: KeyPoint Credit Union, 2805 Bowers Avenue, Santa Clara
RSVP: not required

The measurement, classification and separation and of blood cells have become increasingly important as tools for biological research and clinical diagnostics. ? Blood cells by their very nature have markedly different characteristics. Their character-istics are obtained through differentiation during the normal physical development process of the organism. ? Like all cells, each type of White cell has unique cell surface antigens. ? These can be used to distinguish each type through the use of Fluorescent tagged antibodies to a particular surface antigen characteristic of the cell of interest. ? Flow cytometry can be used to separate the cells into categories of interest by exploiting this technique. ? BD flow cytometers are used for, among other things, the therapeutic monitoring of cancer, AIDS, and leukemia patients. ? For example, the measurement CD4
T-Cells has become a standard in the monitoring and treatment of HIV/AIDS.

This presentation will attempt to describe the various systems BD Biosciences offers for the analysis and sorting of blood cells and the electronic systems employed to make this possible. ? A high-level view of the electronic architecture will be presented. ? Components of the design covered will be: PMT photo detection, multi-channel analog to digital converters, digital delay adjustment for laser beam displacement, calculation of basic cell parameters, spectral overlap compensation, and real-time cell sorting decisions using parallel DSP processing.

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