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Abstract

Abstract: DESCRIPTION (provided by applicant): This proposal describes the feasibility study of using Boston Biomedica, Inc.'s proprietary Pressure Cycling Technology (PCT), its instrument (Barocylcer) and its novel sample preparation tube (PULSE) to process sputum and Mycobacteria species for detection by current laboratory methods. Current sample processing methods are flawed because not only do they reduce the rapid growing native flora but also inactivate most of the viable Mtb, which significantly reduces the sensitivity of growth-based detection methods. Current sample processing methods also require several manipulations by the laboratory worker, which increases their likelihood of exposure to infection through the production of aerosols. If successful, the development of this novel sample processing method will lead to better patient management for tuberculosis by increasing sensitivity leading to earlier detection of the disease. Although beyond the scope of this study, this system may be ultimately combined into an integrated sample preparation procedure and detection system, which is amenable to a high throughput automated format.

Thesaurus Terms:
Mycobacterium tuberculosis, biomedical equipment development, clinical chemistry, communicable disease diagnosis, diagnosis design /evaluation
biomedical automation, sputum, tissue /cell preparation
clinical research, human tissue

Institution:	BIOSEQ, INC.
	217 PERRY PKY
	GAITHERSBURG, MD 20877
Fiscal Year:	2002
Department:
Project Start:	01-AUG-2002
Project End:	31-JUL-2003
ICD:	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG:	ZRG1