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| Grant Number: | 1R43AI052547-01 |
| PI Name: | KARGER, ARIEH M. |
| PI Email: | akarger@rmdinc.com |
| PI Title: | |
| Project Title: | A Simple, High-Speed, Highly Sensitive Viral Sensor |
Abstract: DESCRIPTION (provided by applicant):Radiation Monitoring Devices, Inc. proposes to design a simple, high speed, highly sensitive viral detection instrument. This instrument will measure fluorogenic reporter genes, which hybridize to complementary viral gene target inside a capillary waveguide. Our single-photon sensitive Geiger-mode, microavalanche photodiodes (micro-APDs) will enable low limits of detection of viruses. The micro-APD :an be battery-powered because of its low voltage requirement and rugged characteristics. We will also use active quenching circuitry, which allows measurement of a large dynamic range (-10 E 6), needed for continuous monitoring through exponential nucleic acid amplification. In Phase I, we will design the waveguide sensor, the micro-APD detection system, and establish fluorogenic probe chemistry. In Phase I, we will design a system for viral load detection, array formatting, establish nucleic acid amplification reactions, test a virus, and explore the instrument's versatility.
Thesaurus Terms:
biomedical equipment development, communicable disease diagnosis, fluorescent dye /probe, nucleic acid quantitation /detection, photoconduction, reporter gene, virus DNA
nucleic acid amplification technique, optics, viremia, virus
bioengineering /biomedical engineering
| Institution: | RADIATION MONITORING DEVICES, INC. |
| 44 HUNT ST | |
| WATERTOWN, MA 02472 | |
| Fiscal Year: | 2002 |
| Department: | |
| Project Start: | 01-AUG-2002 |
| Project End: | 31-JAN-2003 |
| ICD: | NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES |
| IRG: | ZRG1 |