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Abstract

Abstract: DESCRIPTION (provided by applicant): Chronic myelogenous leukemia (CML) is characterized by a t(9;22) chromosomal translocation, which results in a fusion gene whose protein product shows enhanced tyrosine kinase activity. This fusion protein is a target in a new promising treatment using a Bcr-Abl kinase inhibitor. CML is typically detected using either slide-based fluorescence in situ hybridization (FISH) to detect the translocation, or reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the fusion mRNA transcript. FISH is labor-intensive and cannot easily detect rare cancer cells. RT-PCR is susceptible to contamination and false positive results. This Phase I research proposes to develop a novel assay format for detecting CML positive cells. Use of flow cytometry will permit analysis of at least a million cells per hour, and identification of rare cancer cells. PROPOSED COMMERCIAL APPLICATION: This assay will facilitate post-treatment patient monitoring and early detection of minimal residual disease (MRD).

Thesaurus Terms:
chronic myelogenous leukemia, cytodiagnosis, diagnosis design /evaluation, flow cytometry, fluorescent in situ hybridization, neoplasm /cancer diagnosis, oligonucleotide, technology /technique development
chromosome translocation, messenger RNA, minimal residual disease
cell line

Institution:	ONE CELL SYSTEMS, INC.
	100 INMAN ST, STE 200
	CAMBRIDGE, MA 02139
Fiscal Year:	2002
Department:
Project Start:	26-AUG-2002
Project End:	28-FEB-2003
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ZCA1