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| Grant Number: | 1R43DK059109-01A2 |
| PI Name: | RUIZ, LEONARD P. |
| PI Email: | lpruiz@attbi.net |
| PI Title: | |
| Project Title: | A hepatocyte spheroid bioassay system for drug discovery |
Abstract: Description (provided by applicant): The liver is the most important organ in metabolizing xenobiotics and drugs. Liver cells (hepatocytes) are used frequently in testing drug metabolism and hepatotoxicity. Hepatocytes in culture can form spheroids that exhibit higher liver-specific functions and sustain high viability longer in culture. This proposed research is to develop an integrated system employing hepatocyte spheroids for both screening purposes and for metabolic studies. In phase I studies, we will develop a high throughput screening system suitable for integration with a miniaturized bioartificial liver for such studies. We will also demonstrate genetic metabolic engineering of hepatocyte spheroids. The analysis of the results will be facilitated by the use of a cDNA microarray immobilized with most relevant DNA sequences. The proposed work will fill a critical need for bioassay systems for high throughput screening. PROPOSED COMMERCIAL APPLICATION: This project will develop an integrated bioassay system using hepatocytes. It will greatly facilitate high throughput screening.
Thesaurus Terms:
bioassay, cell morphology, drug discovery /isolation, liver cell, technology /technique development
biotransformation, drug metabolism, high throughput technology, total artificial organ
biotechnology, microarray technology
| Institution: | ALGENIX, INC. |
| 5264 OXFORD ST N | |
| SHOREVIEW, MN 55126 | |
| Fiscal Year: | 2002 |
| Department: | |
| Project Start: | 15-JUL-2002 |
| Project End: | 31-JAN-2003 |
| ICD: | NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES |
| IRG: | ZRG1 |