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Abstract

Abstract: DESCRIPTION (provided by applicant): It is proposed to develop an inexpensive, scalable, and reproducible photolithographic protein immobilization technology for the fabrication of biosensors and biochips, that will covalently attach proteins to a surface in a uniform orientation, and that will be highly suitable for miniaturization. The technology is based on a light-activated chemistry that will attach proteins to a novel matrix via highly stable covalent bonds. Due to the properties of the reaction, immobilized proteins will maintain their activity and specificity. The matrix will be prepared by attaching reactive groups by silylation. An oligopeptide affinity tag will be isolated from a phage display library that binds to the matrix with high affinity, specifically orientating the protein on the matrix, and forms a covalent bond with the matrix, upon light activation. Because of the immediate compatibility of the chemistry with conventional lithographic methods of chip printing, it will be possible very precisely to control the reaction and limit protein attachment to specific surfaces. Thereby, protein immobilization will become more reproducible, and miniaturization will be facilitated.

Thesaurus Terms:
biosensor, microarray technology, photochemistry, printing, protein, technology /technique development
covalent bond, immobilized enzyme
biotechnology, peptide library

Institution:	AVATAR BIOTECHNOLOGIES, LLC
	NJIT ENTERPRIZE DEV CTR I
	NEWARK, NJ 07102
Fiscal Year:	2002
Department:
Project Start:	01-JUL-2002
Project End:	31-MAR-2003
ICD:	NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
IRG:	ZRG1