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| Grant Number: | 1R43HG002714-01 |
| PI Name: | SCHOENFELD, THOMAS W. |
| PI Email: | tschoenfeld@lucigen.com |
| PI Title: | |
| Project Title: | Improved DNA Polymerases for Genomic Sequencing |
Abstract: DESCRIPTION (provided by applicant): This technology is intended to reduce the costs of sequencing by improving the ability to sequence through difficult templates such as G/C or A/T rich regions, direct and inverted repeats, homopolymer tracts, and di, and tri-nucleotide repeats. This will decrease finishing costs by reducing gaps. Additional improvements will enable direct sequencing of colony derived plasmids and more robust sequencing of BACs, cosmids and genomic DNA. These objectives will be attained by examining an entirely new class of DNA polymerases as improved sequencing reagents.
Thesaurus Terms:
DNA directed DNA polymerase, method development, nucleic acid sequence
bacterial virus, biochemical evolution, thermophilic organism
molecular cloning, protein purification
| Institution: | LUCIGEN CORPORATION |
| 2120 W GREENVIEW DR | |
| MIDDLETON, WI 53562 | |
| Fiscal Year: | 2002 |
| Department: | |
| Project Start: | 27-SEP-2002 |
| Project End: | 26-MAR-2003 |
| ICD: | NATIONAL HUMAN GENOME RESEARCH INSTITUTE |
| IRG: | ZRG1 |