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Abstract

Abstract: DESCRIPTION (provided by applicant) Recent drug discovery efforts have been overwhelmed with numerous new potential drug targets and compounds. Although no single technology will solve this challenge, cell-based analysis will clearly be critical. Existing cell-based assay platforms allow either high-throughput screening (HTS) or high-content screening (HCS), but not both. Oncosis has developed a novel clinical laser-based cell processing system, called PhotosisTM, for high-speed scanning and cell purification via the use of lethal laser effects on unwanted cells. The current Photosis system can scan cells at >105 per second, and can laser-irradiate specific cells leading to various outcomes including death, optoinjection, photobleaching, and molecular uncaging. This system is the foundation for the development of a new research-oriented cell analysis and processing instrument, called LEAPTM, which has the capability to implement HCS assays in a very high-throughput manner (HC/HTS). Feasibility studies are proposed to develop software and run cell-based assays to evaluate the speed of the LEAP platform in HC/HTS. The resulting platform, which also incorporates a targeted treatment laser, will enable new types of drug discovery approaches. Phase II will go on to optimize and implement various cell-based assays in biologically relevant experimental systems, resulting in data supporting this powerful new tool for drug discovery.

Thesaurus Terms:
cell sorting, computer program /software, cytology, high throughput technology, laser, technology /technique development, tissue /cell preparation
computer system design /evaluation, fluorescence spectrometry, fluorescent dye /probe, image processing
bioengineering /biomedical engineering

Institution:	ONCOSIS, INC.
	6199 CORNERSTONE CT, STE 111
	SAN DIEGO, CA 92121
Fiscal Year:	2002
Department:
Project Start:	01-AUG-2002
Project End:	31-JAN-2004
ICD:	NATIONAL CENTER FOR RESEARCH RESOURCES
IRG:	ZRG1