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Abstract

Grant Number: 1R41GM068413-01
PI Name: BORER, PHILIP N.
PI Email: pnborer@orthobiosystems.com
PI Title: PROFESSOR
Project Title: OrthoSwitch Probes for High Throughput Screening

Abstract: DESCRIPTION (provided by applicant): This project aims to develop and test a rapid and sensitive screen for potential anti-nucleocapsid agents. The nucleocapsid (NC) and NC-containing precursor proteins from HIV-1 are targeted for drug discovery. NC is responsible for recognizing and packaging genomic RNA into new virus particles. In addition, the protein participates in other important viral processes, so anti-NC drugs could interfere at several stages of the life cycle of the virus. The screening procedure is a novel application of fluorescence technology, that promises very high sensitivity and selectivity for anti-NC agents. It is capable of being easily scaled for high-throughput screening. The method can also provide an accurate determination of the equilibrium dissociation constants for NC-competitor complexes. In addition, the procedures for developing the fluorescent probes can be simply extended to additional targets in the HIV-1 system, and other pathogens.

Thesaurus Terms:
antiviral agent, drug design /synthesis /production, fluorescent dye /probe, high throughput technology, nucleocapsid, technology /technique development
RNA, intermolecular interaction, virus RNA

Institution: ORTHOSYSTEMS
BOX 762
BRIDGEPORT, NY 13030
Fiscal Year: 2003
Department:
Project Start: 01-APR-2003
Project End: 31-MAR-2004
ICD: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
IRG: ZRG1


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