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Abstract
Grant Number: 1R41GM068413-01 PI Name: BORER, PHILIP N. PI Email: pnborer@orthobiosystems.com PI Title: PROFESSOR Project Title: OrthoSwitch Probes for High Throughput Screening Abstract: DESCRIPTION (provided by applicant): This project aims to develop and test a rapid and sensitive screen for potential anti-nucleocapsid agents. The nucleocapsid (NC) and NC-containing precursor proteins from HIV-1 are targeted for drug discovery. NC is responsible for recognizing and packaging genomic RNA into new virus particles. In addition, the protein participates in other important viral processes, so anti-NC drugs could interfere at several stages of the life cycle of the virus. The screening procedure is a novel application of fluorescence technology, that promises very high sensitivity and selectivity for anti-NC agents. It is capable of being easily scaled for high-throughput screening. The method can also provide an accurate determination of the equilibrium dissociation constants for NC-competitor complexes. In addition, the procedures for developing the fluorescent probes can be simply extended to additional targets in the HIV-1 system, and other pathogens.
Thesaurus Terms:
antiviral agent, drug design /synthesis /production, fluorescent dye /probe, high throughput technology, nucleocapsid, technology /technique development
RNA, intermolecular interaction, virus RNA
Institution: ORTHOSYSTEMS BOX 762 BRIDGEPORT, NY 13030 Fiscal Year: 2003 Department: Project Start: 01-APR-2003 Project End: 31-MAR-2004 ICD: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES IRG: ZRG1
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