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Abstract

Grant Number:	1R43AI051760-01A1
PI Name:	MORROW, PHILLIP R.
PI Email:	pmorrow@avanir.com
PI Title:
Project Title:	Diverse Neutralizing Human Monoclonal Antibodies to CMV

Abstract: DESCRIPTION (provided by applicant): The long-term goal of this proposal (Phase I and II) is to develop a human monoclonal antibody-based preparation for use in treating CMV infections or transmission. Commercial applications for human monoclonal antibodies are extensive. Phase I specific aims are: 1) Screen matching plasma of random buffy coats to identify CMV immune donors, and engraft selected donor cells in SCID mice (hu-PBL-SCID system). 2) Immunize with recombinant gB or gH proteins (neutralizing targets for CMV), and detect specific serum antibodies by ELISA. Optional EBV super-infection may be used. 3) Harvest cells from selected mice, and isolate antigen specific human B/plasma cells by a novel method (antibody rescue technology). Clone heavy and light chain V regions from cells by RT-PCR. 4) Isolate transiently transfected cells producing antigen-specific human monoclonal antibodies. Characterize these for epitope group, affinity, neutralizing capacity, and strain specificity. The main goal of the phase II proposal will be the configuration of at least one combination of human monoclonals for treatment of CMV infection. The phase II SBIR proposal may initially require the leneration of a larger panel of antibodies, based on the success of phase I.

Thesaurus Terms:
cytomegalovirus, immunologic substance development /preparation, monoclonal antibody, neutralizing antibody
recombinant protein
SCID mouse, cell line, enzyme linked immunosorbent assay, human tissue, molecular cloning, polymerase chain reaction, transfection

Institution:	AVANIR PHARMACEUTICALS
	11388 SORRENTO VALLEY RD, STE 200
	SAN DIEGO, CA 921211345
Fiscal Year:	2003
Department:
Project Start:	01-MAY-2003
Project End:	30-APR-2004
ICD:	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG:	ZRG1

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