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Abstract

Grant Number:	1R43AI051766-01A1
PI Name:	PODDAR, SAIBAL K.
PI Email:	poddar1microgen@aol.com
PI Title:
Project Title:	M.pneumoniae Detection by Fluorescence Measurement

Abstract: DESCRIPTION (provided by applicant): Mycoplasma pneumoniae, a human pathogen, primarily causes pneumonia and various other respiratory infections and contributes significantly to worldwide morbidity and mortality. The clinical features associated with M. pneumoniae are often indistinguishable from a variety of other viral and atypical bacterial pathogens, and infections tend to be quite chronic and transmit fast in community-populations. Rapid detection of the microbial agent with high sensitivity and specificity is critical for appropriate treatment and control of disease transmission. The specific focus of this project is to develop a rapid detection method that requires fewer steps to completion, is simple to adopt in a clinical setting, and yet will provide high sensitivity and specificity. The method will be based on single-step amplification of a gene specific of M. pneuminiae in the presence of a stem-loop structured fluorescent energy transfer probe. The amplification will be by a DNA polymerase potentially insensitive to specimen-inhibitor so as to reduce the limitation as applied in clinical samples. The probe will be designed to hybridize specifically with its target; and only upon probe-target hybrid formation, fluorescence will be emitted from a reporter fluorophore attached to it. The presence of M. pneumoniae will be detected by fluorescence measurement of a post-nucleic acid amplified sample immediately after amplification, thereby requiring no additional steps. The method will also be tested in prototype strains and clinical isolates of M. pneumoniae to examine the feasibility of applying it in actual specimens to be tested in Phase II.

Thesaurus Terms:
Mycoplasma pneumoniae, communicable disease diagnosis, diagnosis design /evaluation, fluorescence resonance energy transfer, fluorescent dye /probe, nucleic acid amplification technique, nucleic acid hybridization
DNA primer, nucleic acid probe
clinical research

Institution:	MICROGEN, INC.
	9525 GENESEE AVE, UNIT 216
	SAN DIEGO, CA 92121
Fiscal Year:	2003
Department:
Project Start:	01-JUN-2003
Project End:	30-NOV-2004
ICD:	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG:	ZRG1

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