Version 2.1.1.0

Abstract

Grant Number:	1R43AI054069-01
PI Name:	LARRICK, JAMES W.
PI Email:	calvarez@pano.com
PI Title:	PRESIDENT AND SCIENTIFIC DIRECTOR
Project Title:	A Novel Technology for Molecular Evolution

Abstract: DESCRIPTION (provided by applicant): The post-genomics era will demand high throughput technologies to generate and evolve proteins and protein-binding molecules. The overall goal of this work is to develop a novel ribo-display technology for in vitro molecular evolution. Preliminary work provides proof-of-concept. The next step is to demonstrate the utility and robustness of this novel display system. To attain this goal we will prepare large, highly diverse human scFv libraries and utilize these libraries to identify high affinity antibodies. In phase I we will amplify human antibody sequences to generate a large scFv library and construct ribodisplay vectors. In vitro translation followed by biopanning will be used to identify specific antigen binding scFvs. These will be expressed, purified and characterized by binding and affinity measurements. This simple yet high-yield system should contribute significantly to proteomics research in the post-genome era.

Thesaurus Terms:
directed evolution, genetic translation, peptide library, ribosome, technology /technique development, transfection /expression vector
antibody, immunologic substance development /preparation, protein binding
human tissue, protein purification

Institution:	PANORAMA RESEARCH, INC.
	2462 WYANDOTTE ST
	MOUNTAIN VIEW, CA 94043
Fiscal Year:	2003
Department:
Project Start:	15-MAR-2003
Project End:	14-SEP-2003
ICD:	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG:	ZRG1

---