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Abstract

Grant Number:	1R43AI054150-01
PI Name:	MOEN, PHILLIP T.
PI Email:	phil@onecell.com
PI Title:	SENIOR SCIENTIST
Project Title:	Novel Cytological Assay for HIV

Abstract: DESCRIPTION (provided by applicant): Although advances in antiretroviral therapy, in particular Highly Active Antiretroviral Treatment (HAART) multi-drug regimens, have greatly aided the management of HIV infected patients, treatments cannot permanently eliminate HIV and long-lasting HIV reservoirs persist, primarily in lymphoid tissue. Developing new therapies directed against these reservoirs will require analytical methods that can detect low levels of virus and identify the types of cells harboring residual virus. Rare cell detection is currently a tedious procedure due to the time needed to analyze large numbers of cells. Using fluorescence in situ hybridization and automated image analysis, this SBIR aims to develop a high throughput method for quantifying HIV infected cells present in low frequency. This assay will facilitate rapid diagnosis of HIV infection and development of treatments for HIV infection.

Thesaurus Terms:
HIV infection, biomedical automation, cytodiagnosis, cytology, high throughput technology, image processing, imaging /visualization /scanning, method development, nucleic acid quantitation /detection, phenotype
viremia
bioimaging /biomedical imaging, cell line, fluorescent in situ hybridization

Institution:	ONE CELL SYSTEMS, INC.
	100 INMAN ST, STE 200
	CAMBRIDGE, MA 02139
Fiscal Year:	2003
Department:
Project Start:	15-JAN-2003
Project End:	14-AUG-2003
ICD:	NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
IRG:	ZRG1

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