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Abstract
Grant Number: 1R43CA099411-01 PI Name: HO, WINSTON Z. PI Email: winstonho@maxwellsensors.com PI Title: DIRECTOR, CLINICAL DIAGNOSTIC DEVICES Project Title: Image-coded Microsphere for Multiplexed Bioassay Abstract: DESCRIPTION (provided by applicant): As current research in genomics and proteomics produce more multiplexed data, there is a need for technologies that can rapidly screen a large number of nucleic acids and proteins in a very small volume of sample. Microarray and DNA chips are performed on a planar platform, distributing a small volume of liquid sample over a relatively large chip surface often encounter the problems of an insufficient sample amount or non-uniform distribution. MSI proposes to develop a micro-image-coded microsphere technology for multiplexed thousands of bioassays using an unprecedented level of micro-volume samples. The proposed technology is based on optically bar-coded particles, formed by inserting nano or micro images into a latex bead shell. The novelty of this technology is that image-coded beads identify which reaction is taking place on the surface. Each microbead performs one test, thus acting as a single analyte analyzer. By adding a mixture of micro particles, several thousands analytes can be tested simultaneously, easily, rapidly, and inexpensively. Phase I work will focus on image-coded particle synthesis, micro image recording, optical decoding, and DNA hybridization assays. In Phase II, the technology will be applied to cellular analysis, single nucleotide polymorphisms (SNP), and DNA-based cancer diagnostics.
Thesaurus Terms:
bioassay, high throughput technology, holography, imaging /visualization /scanning, microarray technology, microcapsule, technology /technique development
functional /structural genomics, nanotechnology, proteomics
biotechnology, nucleic acid hybridization
Institution: MAXWELL SENSORS, INC. 10020 PIONEER BLVD, STE 103 SANTA FE SPRINGS, CA 906706213 Fiscal Year: 2003 Department: Project Start: 03-FEB-2003 Project End: 02-AUG-2003 ICD: NATIONAL CANCER INSTITUTE IRG: ZRG1