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Abstract

Grant Number:	1R43CA101274-01
PI Name:	ZELTSER, GREGORY
PI Email:	sutama@poc.com
PI Title:	RESEARCH-SCIENTIST
Project Title:	Microchannel Proteomic Array

Abstract: DESCRIPTION (provided by applicant): Physical Optics Corporation (POC) proposes to design, develop, fabricate, and test a Microchannel Proteomic Array (MPA) system based on proprietary silicon chip fabrication technology and integration of immunodetection amplified by T7 RNA polymerase (IDAT) and fluorescence-in-situ-hybridization (FISH) techniques. The system will provide high throughput, precise isolation, and quantitative analysis of proteins in unpurified samples of body fluids and/or cancer cells, as well as allow carrying out protein-protein and protein-ligand interaction studies. The MPA system will consist of three components: a microarray chip, a POC backlit holographic diffuser screen, and a processing/analyzing subsystem. The chip will have up to 10,000 micro-channels on a 2. 25 cm2 plate surface and the potential to simultaneously assay ten thousand proteins/ oligopepetides in an unpurified sample in hours. The highly sensitive chip combined with a massively parallel, automated analysis array can yield ultrahigh throughput. The microchip will consume only nanoliters of the sample. In Phase I, POC will investigate micro-channel proteomic array system design and architecture to demonstrate proof-of-concept.

Thesaurus Terms:
high throughput technology, protein protein interaction, protein quantitation /detection, proteomics, technology /technique development
biomedical automation, charge coupled device camera, intermolecular interaction, ligand, peptide library, protein structure function
biotechnology

Institution:	PHYSICAL OPTICS CORPORATION
	20600 GRAMERCY PL, BLDG 100
	TORRANCE, CA 905011821
Fiscal Year:	2003
Department:
Project Start:	01-APR-2003
Project End:	30-SEP-2003
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ZRG1

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