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Abstract

Grant Number:	1R43CA101443-01
PI Name:	BLAND, KIMBERLY S.
PI Email:	kbland@virtualarrays.com
PI Title:
Project Title:	Cell Arrays Generated by a Novel Transfection Method

Abstract: DESCRIPTION (provided by applicant): To address the rising need for cell assays in drug discovery, we propose a method for the rapid and simple generation of cell arrays by incorporating a novel transfection method into our current cell array technology. Our technology platform uses encoded microcarriers to multiplex pre-established cell types for simultaneous interrogation in a single microtiter well. We propose to combine our platform with a method of "reverse transfection" in which cells become transfected with material pre-deposited on a substrate. Specifically, we propose to use the carriers to deliver transfection material to cells plated on top of them. This approach would enable drug discovery laboratories to perform cell assays in a multiplexed manner, thereby decreasing the time, labor and reagent use involved with these assays as well as increasing the quality of data obtained from them. In addition, using reverse transfection to generate cell arrays substantially decreases the tissue culture burden associated with traditional transfection by alleviating the need for separate transfections and stable cell lines. We have obtained preliminary data supporting the technical feasibility of reverse transfection using encoded carriers. In this proposal, we outline a strategy for optimizing this approach and testing its feasibility for use in multiplexed cell assays.

Thesaurus Terms:
cell population study, cytogenetics, high throughput technology, technology /technique development, tissue /cell preparation, transfection
tissue /cell culture
biotechnology

Institution:	VIRTUAL ARRAYS, INC.
	1190 BORDEAUX DR
	SUNNYVALE, CA 94089
Fiscal Year:	2003
Department:
Project Start:	01-APR-2003
Project End:	31-MAR-2004
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ZRG1

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