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Abstract

Grant Number:	1R43CA103085-01
PI Name:	SCHNEIDER, LUKE V.
PI Email:	luke_schneider@targetdiscovery.com
PI Title:
Project Title:	IDBEST Differential Display on Protein Chips

Abstract: DESCRIPTION (provided by applicant): Differential display analysis on DNA and protein chips has proven useful in the identification of biomarkers for cancer. Isotope tagging methods, such as ICAT, have the potential to significantly improve the precision of these methods, but cannot currently be applied to protein chips where the tagged peptides cannot be effectively separated from untagged peptides. However, by incorporating one of the elements with atomic numbers between 35 (Br) and 63 (Eu) into isotopic tags, the resulting tagged peptides are shifted in the mass spectrum by 0.1 amu. As our preliminary work shows, these isotope differentiated binding energy shift tags (IDBEST) allow direct detection of the tagged peptides from untagged species in the mass spectrum without the need for affinity or liquid chromatography clean up of the sample. The goal of this project is to develop isotope differentiated binding energy shift tags (IDBEST) and associated mass spectral filtering algorithms that allow high precision (<10% standard deviation) differential display to be conducted directly on affinity protein chips.

Thesaurus Terms:
chemical binding, differential display technique, microarray technology, protein quantitation /detection, technology /technique development
biomarker
biotechnology, cell line, clinical research, human genetic material tag

Institution:	TARGET DISCOVERY
	401 FABIAN WAY
	PALO ALTO, CA 94303
Fiscal Year:	2003
Department:
Project Start:	01-JUL-2003
Project End:	30-JUN-2004
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ZCA1

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