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Abstract

Grant Number:	1R43CA103335-01
PI Name:	SULLIVAN, BRIAN J.
PI Email:	bsullivan@kumetrix.com
PI Title:
Project Title:	Cancer Detection via Enzymatic Microassays

Abstract: DESCRIPTION (provided by applicant): New methods for easy, rapid enzymatic assays in very small sample volumes (sub-microliter) will greatly benefit the prognosis, diagnosis and detection of cancer. The goal of Phase I is to demonstrate the feasibility of adapting silicon microsampling and assay devices originally developed for blood glucose monitoring, to enzyme activity measurement using a novel technique, "Diffusive Enzyme Activity Measurement." An electrochemical redox-mediated prostatic acid phosphatase (PAP) assay will be the example, requiring no on-chip biologicals (providing a potentially long shelf-life). Silicon microchips with the novel diffusional microfluidics will be designed and fabricated. A computer simulation model will be used in the development of the assay. The enzyme chosen for Phase I is a known cancer marker for prostate cancer. Potential commercial applications include enzyme activity measurements on sub-microliter liquid samples, such as blood drawn from experimental animals (or humans), tissue extract specimens, purified enzymes and drug discovery research and development.

Thesaurus Terms:
diagnosis design /evaluation, enzyme activity, neoplasm /cancer diagnosis
biomarker, diffusion, electrochemistry, microprocessor /microchip, prostate neoplasm, protein tyrosine phosphatase, silicon
computer simulation

Institution:	KUMETRIX, INC.
	29524 UNION CITY BLVD
	UNION CITY, CA 94587
Fiscal Year:	2003
Department:
Project Start:	01-JUL-2003
Project End:	31-DEC-2003
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ZRG1

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