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Abstract

Grant Number:	1R43DK063837-01
PI Name:	LASKEN, ROGER S.
PI Email:	rogerl@molecularstaging.com
PI Title:
Project Title:	Affordable large population genomic DNA collections

Abstract: DESCRIPTION (provided by applicant): The availability of large human genomic DNA sets of known ethnic origin remains a persistent problem for epidemiology and disease gene research. In this grant two valuable resources are combined to solve DNA availability problems. Molecular Staging Inc (MSI) has developed a technology, termed Multiple Displacement Amplification (MDA), that is capable of producing 106 fold amplification of whole human genomes that faithfully represent the original templates, with excellent representation of genetic loci and high fidelity of sequence representation. This technology will be used to amplify an extensive set of archived human genomic DNA samples of defined ethnic origin, representing Asian, African American and Caucasian populations, previously collected by Dr. Beutler of Scripps Research Institute during a project funded by NIDDK. These DNA samples will be amplified and arrayed using bar code controlled automated processes and will be provided to other NIH funded researchers. In Phase I of this SBIR we will set up automation and quality control of amplification and arraying processes.

Thesaurus Terms:
genetic registry /resource /referral center, genome, molecular biology information system, nucleic acid amplification technique, technology /technique development
biotechnology, clinical research, human genetic material tag

Institution:	MOLECULAR STAGING, INC.
	300 GEORGE ST, 7TH FL
	NEW HAVEN, CT 06511
Fiscal Year:	2003
Department:
Project Start:	01-APR-2003
Project End:	30-SEP-2004
ICD:	NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
IRG:	ZRG1

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