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Abstract

Grant Number:	1R43GM068177-01
PI Name:	BROWN, MARK J.
PI Email:	mbrown@echelon-inc.com
PI Title:
Project Title:	Intracellular Localization of Protein Prenylation

Abstract: DESCRIPTION (provided by applicant): Isoprenoid diphoshates are key metabolic intermediates for the biosynthesis of many classes of molecules required by single and multicellular organisms. They fulfill a wide variety of cellular functions: components of cell membranes (sterols), electron transport (ubiquinones), signal transduction (prenylated proteins), and cell wall biosynthesis (dolichols)-essential for viability. A detailed understanding of the mechanisms by which isoprenoids elicit their effects has been limited by the difficulty of getting isoprenoid diphosphates into live cells. Echelon Biosciences, Inc. will develop a simple new method for delivering isoprenoid diphosphates and/or their precursors into cells passively and without disruption of cellular integrity. Two methods will be used, either independently or in tandem to accomplish this task. In the first approach, we will test the feasibility of delivering isoprenoid monophosphate and derivatives into cells, which then can be converted by an intracellular kinase to the diphosphates. The second approach will employ shuttle molecules that will be developed based on prior expertise at Echelon for the intracellular delivery of phosphoinositides.

Thesaurus Terms:
isoprenoid, membrane permeability, method development, phosphate, posttranslational modification, protein localization, transferase
geranyl compound, intracellular transport
cell line, chemical synthesis, fluorescence microscopy, transfection

Institution:	ECHELON BIOSCIENCES, INC.
	675 ARAPEEN DR, STE 302
	SALT LAKE CITY, UT 84108
Fiscal Year:	2003
Department:
Project Start:	01-JUL-2003
Project End:	30-JUN-2004
ICD:	NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
IRG:	ZRG1

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