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Abstract
Grant Number: 1R43RR018420-01 PI Name: SU, XIAO-LI PI Email: xsu@virtual-incubation.com PI Title: Project Title: A High-Performance Food Pathogen Detection Instrument Abstract: DESCRIPTION (provided by applicant): Food safety remains a critical issue for human health in our society. The food system in our nation is vulnerable to contamination from natural pathogens and from bio-terrorist attack. Current detection methods are inadequate due to the time-consuming and laboratory-centered approaches presently in use. Instruments for rapid on-site detection of pathogens in food are urgently needed. The objective of this project is to evaluate an innovative capillary bioseparator / bioreactor-based optical biosensor technology for rapid detection of E. coli O157:H7. The detection limit, specificity and detection time possible with this approach will be evaluated. It is expected that the technology will provide <100 cells/ml detection limit, <1 h detection time, and high specificity to E. coli O157:H7 (<1% false positive/negative data). Although the initial work will focus on food safety, similar technology can be utilized for rapid, on-site screening of human E. coli infections. Rapid screening of human infections is also very important because life-threatening E. coli infections are increasing especially in susceptible groups such as premature babies. Preliminary data indicate that the proposed approach has good potential to significantly improve the state-of-the-art in rapid, sensitive, and specific detection of E. coli O157:H7 and eventually other biological pathogens.
Thesaurus Terms:
biohazard detection, bioreactor, food contamination, technology /technique development
Escherichia coli 0157:H7, antigen antibody reaction, bacteria infection mechanism, bacterial food poisoning, biosensor, food quality /standard, optics
Campylobacter, Listeria, Salmonella typhimurium, biotechnology, spectrometry
Institution: BIODETECTION INSTRUMENTS, INC. 21 W MOUNTAIN, STE 122 FAYETTEVILLE, AR 72701 Fiscal Year: 2003 Department: Project Start: 30-SEP-2003 Project End: 29-MAR-2004 ICD: NATIONAL CENTER FOR RESEARCH RESOURCES IRG: ZRG1