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Abstract

Grant Number:	1R43RR018647-01
PI Name:	BERKOUT, VADYM D.
PI Email:	vberkout@apmaldi.com
PI Title:
Project Title:	New Method of Ion Dissociation in QTOF Mass Spectrometry

Abstract: DESCRIPTION (provided by applicant): The main goal of this proposal is to develop a novel method of fragmentation of biomolecules in an electrospray ionization (ESI) quadrupole/time-of-flight mass spectrometer (Q-TOF MS). The new method will enable many more bonds to cleave than a conventional collisionally activated dissociation. It also will preserve labile modifications of the amino acid side chains, such as phosphorylation and glycosylation that facilitates determination of their sites, and can also distinguish between Leucine and Isoleucine amino acid residues. Combined with liquid separation techniques (HPLC,CE) Q-TOF mass spectrometer with a new fragmentation method will provide high-throughput analysis of protein mixtures.

Thesaurus Terms:
electrospray ionization mass spectrometry, mass spectrometry, method development, posttranslational modification
glycosylation, ion cyclotron resonance spectrometry, phosphorylation
capillary electrophoresis, high performance liquid chromatography

Institution:	SCIENCE AND ENGINEERING SERVICES, INC.
	4032 BLACKBURN LN
	BURTONSVILLE, MD 20866
Fiscal Year:	2003
Department:
Project Start:	01-AUG-2003
Project End:	31-JAN-2004
ICD:	NATIONAL CENTER FOR RESEARCH RESOURCES
IRG:	ZRG1

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