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Abstract
Grant Number: 2R44EB000560-02A2 PI Name: HO, WINSTON Z. PI Email: winstonho@maxwellsensors.com PI Title: DIRECTOR, CLINICAL DIAGNOSTIC DEVICES Project Title: Chemiluminescence Biochip for Immunoassay Abstract: DESCRIPTION (provided by applicant): Current integrated modular systems for clinical diagnostics only operate after a large collection of sample material. Unfortunately, this increases the amount of time for patients to receive their test results or physicians to plan a course of treatment. Present day point-of-care-testing (POCT) immunoassay systems provide rapid results, but they are known for their poor accuracy and sensitivity, and often limited to qualitative or semi-quantitative assays. MSI has proposed to develop a portable, integrated, POCT system, by combining a self-contained, miniaturized chemiluminescence chip (CLCHIP) and microfluidic technologies that will dramatically improve POCT accuracy, sensitivity, and reliability. This innovative CLCHIP platform utilizes microfabrication technology to automate microfluidics for multi-analyte and multi-step biochemistries and reduce a complicated procedure to a single step. During Phase I of this project, we designed and fabricated microfluidic biochips with patterned microchannels, developed an on-chip chemiluminescence immunoassay protocol, performed a luteinizing hormone and troponin I bioassay, obtained linear dose-response curves, and achieved a total assay time of 15 minutes. Based on the foundation of Phase I we will move into Phase II, in order to optimize CLCHIP development, complete system integration, perform various analyte assays, multi-analyte panel testing, and achieve clinical performance requirements.
Thesaurus Terms:
biomedical equipment development, diagnosis design /evaluation, immunologic assay /test, luminescence, microarray technology
chemical reaction, estradiol, fluid flow, luteinizing hormone, miniature biomedical equipment, parathyroid hormone, portable biomedical equipment, troponin
human tissue
Institution: MAXWELL SENSORS, INC. 10020 PIONEER BLVD, STE 103 SANTA FE SPRINGS, CA 906706213 Fiscal Year: 2003 Department: Project Start: 01-JUL-1999 Project End: 31-MAR-2005 ICD: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING IRG: ZRG1