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Abstract

Grant Number:	2R44RR016833-02
PI Name:	NISHIOKA, GARY M.
PI Email:	hni@infinet.com
PI Title:
Project Title:	Vitrified Immobilized Proteins and Ligands

Abstract: DESCRIPTION (provided by applicant): The overall objective of this research is to develop a new process and laboratory instrument capable of synthesizing microspots of vitrified immobilized proteins and ligands. Immobilized proteins and ligands stored in a vitrified (glassy) sugar matrix would be well preserved at room and even elevated temperatures. The ability to create such microspots could generate significant improvements in performance, cost, convenience, and storage requirements for applications requiring immobilized proteins and ligands. Examples of applications that could be improved include immunoassays, syntheses using immobilized enzymes, purifications involving affinity chromatography, and sensors using immobilized antibodies, enzymes, and ligands.

Thesaurus Terms:
biomedical equipment development, immobilized enzyme, microarray technology, peptide chemical synthesis, preservation, technology /technique development
affinity chromatography, enzyme linked immunosorbent assay, ligand, protein purification, protein structure function
biotechnology

Institution:	H AND N INSTRUMENTS, INC.
	219 N WESTMOOR AVE
	NEWARK, OH 43055
Fiscal Year:	2003
Department:
Project Start:	01-FEB-2002
Project End:	30-APR-2005
ICD:	NATIONAL CENTER FOR RESEARCH RESOURCES
IRG:	ZRG1

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