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Abstract

Grant Number:	4R44HL072671-02
PI Name:	NELSON, RANDALL W.
PI Email:	rnelson@intrinsicbio.com
PI Title:	PRESIDENT & CEO
Project Title:	Mass Spectrometric Immunoassay of Apolipoproteins

Abstract: DESCRIPTION (provided by applicant): Proposed in this Fast Track Phase I/Phase II proposal is the development of mass spectrometric immunoassays (MSIA) targeting apolipoproteins in human blood. Assays, utilizing antibody-derivatized affinity pipettes and MALDI-TOF mass spectrometry, will be developed for Apo-A, -C, -D, -E, -H and -J. These assays will have general use in studies investigating the quantitative modulation of, and point mutations and posttranslational modifications present in, the apolipoproteins. Phase I research will address the Specific Aim of optimizing affinity pipette chemistries for apolipoproteins. By reaching the Phase I milestone of high reproducibility, low non-specific binding qualitative assays (for the apolipoproteins when analyzed from small volumes (< 50 uL) of human blood), we will proceed into Phase II research. Phase II objectives include: 1) The use of enzymatically-active MALDI-TOF MS targets in conjunction with MSIA for structural characterization of the apolipoproteins, 2) The incorporation of quantitative methodologies into the assays, and 3) The development of a MSIA assay for common ApoE phenotypes. The final objective of the program is the development of kits and low-cost analytical platforms for the high-throughput analysis of apolipoproteins. Upon supply to the public, the approach will find initial applications in proteomic investigations linking protein variants with disease and potential long-term applications as phenotyping assay for use in clinical/diagnostic or drug administration applications.

Thesaurus Terms:
apolipoprotein, immunologic assay /test, matrix assisted laser desorption ionization, method development
apolipoprotein E, point mutation, posttranslational modification, protein binding, proteomics
biotechnology, clinical research, human subject

Institution:	INTRINSIC BIOPROBES, INC.
	625 S SMITH RD, STE 22
	TEMPE, AZ 85281
Fiscal Year:	2003
Department:
Project Start:	17-JAN-2003
Project End:	30-JUN-2005
ICD:	NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
IRG:	ZRG1

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