Version 2.1.1.0

Abstract

Grant Number:	5R43CA001790-02
PI Name:	BROUSSEAU, LOUIS C.
PI Email:	lou@quantumlogicdevices.com
PI Title:
Project Title:	Single DNA Sequencing with Tunneling Spectroscopy

Abstract: DESCRIPTION (provided by applicant): This research will create a new technique, capable of direct sequencing of single molecules of RNA, DNA and other oligonucleotides. By combining ultra sensitive quantum dots with scanning probe microscopy, molecular topology is recorded simultaneously with chemical composition at sub-nanometer resolution. This enables determination of the nucleoside sequence as well as the identification of single nucleotide polymorphisms, kinks, dislocations, and other mutations of the strand. The prototype and developed software will allow high throughput and automated sample analysis. This novel tool will be enabling for the realization of genetic-based healthcare (pharmacogenomics). By providing a direct, non-PCR method for genetic sequencing, routine application of this information to genetic predisposition screening, diagnosis, and prescription becomes available for clinical laboratories and hospitals. Expression and mutation studies can also be applied to drug development, as well as for more effective clinical trial candidate selection.

Thesaurus Terms:
image enhancement, nucleic acid sequence, nucleic acid structure, quantum chemistry, scanning tunneling microscopy, technology /technique development
computer assisted sequence analysis, computer program /software, genetic polymorphism, high throughput technology, nanotechnology
bioimaging /biomedical imaging

Institution:	QUANTUM LOGIC DEVICES
	7801 N LAMAR, STE D-98
	AUSTIN, TX 78752
Fiscal Year:	2003
Department:
Project Start:	01-SEP-2002
Project End:	31-AUG-2004
ICD:	NATIONAL CANCER INSTITUTE
IRG:	ZRG1

---