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Abstract
Grant Number: 5R44HG002327-03 PI Name: DUGAS, MATTHEW PI Email: mdugas@gotoarc.com PI Title: Project Title: Rapid, Single Molecular DNA Sequencing Nanopore Device Abstract: DESCRIPTION (provided by applicant): The objective of this Phase II program is to complete the development of a commercializable solid state nanopore device for the accurate high speed sequencing of DNA. To achieve this goal a manufacturable device must be designed and fabrication processes established that result in a unit with extremely small, well controlled diameter orifices of approximately 2 nm through a multi-layered thin film sandwich of insulators and conductors. The Phase I portion of this program established the methodology of: creating a thin (30 nm) SiN film over a 20 um square window in a 1 cm square silicon frame; Focused Ion Beam (FIB) milling a nanopore; subsequent deposition of a multi-layer thinfilm sandwich of conductor and insulating layers on the surface of SiN film; and building a cartridge to hold and seal the nanopore chip between two liquid cells, and provide electrical terminations for measuring the changes in current, potential, or capacitance resulting in the passage of a DNA molecule chain through the nanopore. It was found that FIB milling produced a nanopore that was too wide and lacked the required sensitivity to map DNA molecules passing through the nanopore.. A new approach, TEM drilling will be used to achieve nanopores of 2 nm or smaller. Additional work will be invested in optimizing the leads and instrumentation, i.e., patch clamp amplifiers to achieve improved signal-to-noise ratios and increased detection sensitivity.
Thesaurus Terms:
biomedical automation, biomedical equipment development, high throughput technology, nanotechnology, nucleic acid quantitation /detection, nucleic acid sequence
microelectrode, molecular film, semiconduction, silicon compound
bioengineering /biomedical engineering, electrophoresis, transmission electron microscopy, voltage /patch clamp
Institution: ADVANCED RESEARCH CORPORATION 815 14TH AVE SE MINNEAPOLIS, MN 55414 Fiscal Year: 2003 Department: Project Start: 28-SEP-2001 Project End: 31-AUG-2004 ICD: NATIONAL HUMAN GENOME RESEARCH INSTITUTE IRG: ZRG1