| Version 2.5.2.0 |
|
|
Abstract
Grant Number: 1R43CA126647-01 Project Title: ENZYMATIC LUMINESCENCE microRNA ASSAY
PI Information: Name Title SUN, YE ye_sun@scien-tec.com Abstract: DESCRIPTION (provided by applicant): The goal of this project is to develop a new, highly sensitive, and cost-effective RNA Enzymatic Luminescence Assay (qELA) for high-throughput detection and quantification of microRNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expands pyrosequencing detection methodology for highly sensitive and accurate quantification of small RNA molecules. The proposed assay has unique sensitivity and dynamic range and is expected to outperform real-time PCR in applications for analysis of small RNA molecules. The qELA requires less expensive reagents and equipment than RT-PCR and microarrays and can be used in a number of commercial assays for application in life sciences research, drug discovery, and clinical diagnosis.
Thesaurus Terms:
There are no thesaurus terms on file for this project.
Institution: SCI-TEC, INC. #300 KNOXVILLE, TN 37932 Fiscal Year: 2007 Department: Project Start: 01-MAY-2007 Project End: 30-APR-2009 ICD: NATIONAL CANCER INSTITUTE IRG: ZCA1