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Abstract
Grant Number: 1R43EB006249-01A1 Project Title: High Efficiency Transfection via Nanospearing
PI Information: Name Title CAI, DONG dcbio2002@yahoo.com Abstract: DESCRIPTION (provided by applicant): NanoLab is proposing to develop a new biomolecule delivery technique named "nanospearing", based on its previously published work. DNA plasmids are immobilized on magnetically susceptible, needle-like nanostructures. These needles can be magnetically accelerated to penetrate ('spear') the cell membrane. The plasmid DNA bound to the nanotubes can be released and expressed in the cells. Two pieces of equipment, called GeneBlender and GeneHammer, are proposed for spearing cells in suspension and on substrates respectively. Theoretical analysis is to to be used for optimizing the system configuration so that nanotubes can be driven to their highest speed for spearing. Different molecular immobilization strategies will be tested to achieve the efficient plasmid loading. Upon ressolving the potential pitfalls, the process will be universalized and packaged into a transfection kit. The development will be focused on plasmid DNA delivery at this phase. It is enasaged for applications in vivo, such as drug delivery and vaccination.
Thesaurus Terms:
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Institution: NANOLAB, INC. 55 CHAPEL ST NEWTON, MA 02458 Fiscal Year: 2007 Department: Project Start: 01-JUL-2007 Project End: 30-JUN-2009 ICD: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING IRG: ZRG1