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Abstract
Grant Number: 1R43HG003417-01A1 Project Title: Rapid Screening of Nucleic Acid Energetics
PI Information: Name Title PLUM, GEORGE ERIC. eplum@ibetinc.com PRESIDENT Abstract: DESCRIPTION (provided by applicant): Project Summary/Abstract: Recent advances, perhaps most visible in the Human Genome Initiative, have presented new and compelling means and objectives to exploit the exquisite specificity of nucleic acid hybridization. Precise knowledge of the temperature dependent stability of nucleic acid (and nucleic acid analog) complexes is necessary for the rational design and optimization of hybridization probes for use in high throughput screening, nucleic acid based diagnostics and therapeutics, and numerous emerging biotechnology applications. The primary objective of the proposed project is to develop a high-throughput means of obtaining accurate thermal and thermodynamic data for nucleic acid complexes by measurement and analysis of optically-monitored, temperature-induced, order-disorder transitions. The proposed device will operate analogously to a pH meter, the operator will load a nucleic acid complex sample and after a short settling time a numerical result will be displayed. The device will provide, in a matter of seconds, a complete thermodynamic analysis capable of predicting accurately the stability of the nucleic acid complex at any temperature. Project Narrative: The primary objective of the proposed project is to develop a high-throughput means of obtaining accurate thermal and thermodynamic data for nucleic acid complexes by measurement and analysis of optically-monitored, temperature-induced, order-disorder transitions. This information is useful in designing and troubleshooting probes and in more fundamental studies of nucleic acids.
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Institution: IBET, INC. 1507 CHAMBERS RD, STE 301 COLUMBUS, OH 432121568 Fiscal Year: 2006 Department: Project Start: 20-SEP-2006 Project End: 28-FEB-2008 ICD: NATIONAL HUMAN GENOME RESEARCH INSTITUTE IRG: ZRG1