Abstract
| Grant Number: | 5R44ES013805-03 |
| Project Title: | Targeted Antibody Microarrays:Tool for Toxicoproteomics |
| PI Information: | Name | Email | Title |
| KIM, HYESOOK |
hskim@aol.com |
PRINCIPAL INVESTIGATOR /PRESIDENT |
Abstract: DESCRIPTION (provided by applicant): Gene expression has been analyzed by throughput DMA microarrays which simultaneously monitor the expression of thousands of genes. However, mRNA expression of a gene may not correlate with protein expression because of the differences in rates of mRNA translation/degradation and post-translational modification. Mass spectrometry with/without prior separation of proteins has been developed but the burden of the experiment is on the user.
During Phase I, we successfully developed and commercialized rat drug-metabolizing enzyme antibody microarrays containing 76 antibodies for cytochromes P450 and co-enzymes, phase II drug-metabolizing enzymes, apoptosis-related proteins, house-keeping proteins and Flag internal control protein.
During Phase II, we will (1) further improve rat polyacrylamide-based hydrogel arrays, (2) improve and develop additional arrays with chips coated with various surface materials, (3) develop and explore signal enhancing methods, (4) develop mouse drug-metabolizing enzyme antibody microarrays, (5) develop human drug-metabolizing enzyme antibody microarrays and (6) explore utility of antibody arrays and discover markers for 5 classes of toxicants.
The targeted arrays produced with form-specific antibodies of closely related proteins involved with drug and environmental toxicant metabolism will be an invaluable tool for toxicoproteomics.
Thesaurus Terms:
antibody specificity, drug metabolism, environmental toxicology, method development, microarray technology, proteomics, toxin metabolism
biological signal transduction, biomarker, enzyme activity, gel, gene expression, polyacrylamide, surface coating, toxicant screening
SDS polyacrylamide gel electrophoresis, laboratory mouse, laboratory rat, western blotting
| Institution: | DETROIT R & D, INC. |
| 2727 SECOND AVE., SUITE 4113 |
| DETROIT, MI 48201 |
| Fiscal Year: | 2006 |
| Department: | |
| Project Start: | 01-MAR-2005 |
| Project End: | 31-AUG-2008 |
| ICD: | NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES |
| IRG: | ZRG1 |
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