Abstract
| Grant Number: | 5R44HL065977-03 |
| Project Title: | MEMBRANE BASED ROLLER BOTTLE |
Abstract: DESCRIPTION (provided by applicant): The culture of cells is currently undertaken to produce small and large quantities of cells and cell-secreted products, such as growth factors, enzymes, hormones, monoclonal antibodies, interferons, interleukins, vaccines, diagnostics, and viral vectors. A variety of commercially available membrane-based cell culture devices are available for the culture of mammalian ceils that have been adapted to suspension culture. A growing number of industrially important cells, however, are anchorage-dependent. Existing membrane-based devices lack the proper materials for adherent cell attachment and have sub-optimal surface areas. The membrane based roller bottle (MBRB) is a practical, cost effective culture device designed to meet this need and improve culture productivity. The MBRB combines the simplicity of the traditional roller bottle with membrane-based attributes. In the proposed Phase II studies, the MBRB will be optimized for the production of anchorage-dependent cells, cell-secreted products, and cellular transduction. Specific aims include a thorough mechanical design investigation followed by on-site and off-site application testing. The commercialization of a membrane-based family of research and clinical scale roller bottle devices is expected to lead to a more efficient use of biotechnology resources. At the completion of Phase II, the MBRB will be targeted to manufacturers of small and mid-scale culture devices for the production of cells or cell products for diagnostic and therapeutic applications.
Thesaurus Terms:
artificial membrane, biomedical equipment development, tissue /cell culture
consumable /disposable biomedical equipment, growth media, mass tissue /cell culture
enzyme linked immunosorbent assay, flow cytometry
| Institution: | WILSON WOLF MANUFACTURING CORPORATION |
| SUITE 700 |
| NEW BRIGHTON, MN 55112 |
| Fiscal Year: | 2004 |
| Department: | |
| Project Start: | 15-SEP-2000 |
| Project End: | 30-JUN-2008 |
| ICD: | NATIONAL HEART, LUNG, AND BLOOD INSTITUTE |
| IRG: | ZRG1 |
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